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. 2016 Sep 26;73(11):680–690. doi: 10.1002/cm.21336

Figure 2.

Figure 2

LARP4 depletion increases cell migration. (A) PC3 cells were transfected with a pool of LARP4 siRNA2 and LARP4 siRNA4 or control siRNA. Cells were seeded onto Matrigel‐coated wells with stoppers. After 24 h, stoppers were removed, and images of the wells were acquired (t = 0) and 24 h later (t = 24). Values represent the mean migration into the gap left by the stopper as a percentage of the control ± SEM; n = 3 independent experiments (4 technical replicates/experiment); one‐tailed, two‐sample t‐test; *P < 0.05. (B–E) PC3 and MDA‐MB‐231 cells were transfected with control siRNA or siRNAs targeting LARP4. Images of cells, 72 h after siRNA transfection, were captured at 1 frame/10 min for 16 h by time‐lapse microscopy. (B–C) Plots show example tracks of 20 PC3 (B) or MDA‐MB‐231 cells (C) from one of three independent experiments. (D–E) Mean migration speed (μm/min) of PC3 (D) and MDA‐MB‐231 cells (E); n = 88 cells (≥ 18 cells per experiment) from three independent experiments for PC3 cells, and n = 142 (≥44 cells per experiment) from three independent experiments for MDA‐MB‐231 cells. Values represent the migration speed of each cell as a dot and the median with 25th and 75th percentiles; one‐way ANOVA followed by Tukey's multiple comparisons test; ****P < 0.0001.