Figure 4.

LARP4 depletion causes cell elongation in 3D. MDA‐MB‐231 cells were transfected with control siRNA or siRNAs targeting LARP4. (A) 3D morphology assay in Matrigel. 48 h after siRNA transfection, cells were embedded in Matrigel and seeded onto Matrigel‐coated 96‐well plates. Example images are shown. Scale bar: 100 μm. (B) Cells were assigned a shape score from 0 to 3, as indicated on the image for LARP4‐2 siRNA and in the enlarged image below (Cell elongation scoring): a score of 0 indicates a round cell and a score of 1, 2 or 3 indicates cells with progressively longer protrusions. Graph shows mean elongation scores of cells for each condition +/‐ SEM; n =3 independent experiments (4 images per experiment per condition).