Figure 2.

Spatial reconstruction of single cell gene expression data using in situ sequencing and scRNA‐seq. A: In situ sequencing is performed on tissue of interest to generate gene expression data that are used to profile different cell types. B: Single cell obtained from a tissue with the same origin (same morphology) or from a consecutive section are subjected to scRNA‐seq. After profiling the scRNA‐seq data, cells that match the in situ sequencing gene expression patterns are placed to their corresponding positions to reconstruct the spatial organization of the tissue.