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. 2016 Jul 25;59(12):773–780. doi: 10.1111/myc.12530

Table 1.

Primers and probe for the real‐time PCR assay

Primers and probe Nucleotide sequencesa Position of DQ08822 Tm (°C)
Forward primer 5′‐TCTGGACGGYGTTCAGTC‐3′ 222–239 57
Reverse primer 3′‐TGATTGCTTAAATCCTGAACA‐5′ 294–314 52
Probe 5′‐FAM‐AAAATGAGCCTCCGCTTA
GCTCCATGG‐BHQ1‐3′ 		249–271 52
a

Y = T or C; 5′‐FAM = 5′ 6‐carboxyfluorescein; BHQ, black hole quencher.