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. Author manuscript; available in PMC: 2016 Nov 16.
Published in final edited form as: Cell Rep. 2016 Oct 4;17(2):336–344. doi: 10.1016/j.celrep.2016.09.028

Figure 3. rh5- and rh6-dependent temperature selection depends on trpA1-neurons, but not the Bolwig organ.

Figure 3

(A) Staining of control (w1118) larvae with anti-Rh6. The antibodies labeled neurons in the Bolwig organ (arrows) and their processes that extend to the brain (arrowheads). The scale bars in A and B represent 100 μm.

(B) Anti-Rh6 staining was not detectable after elimination of photoreceptor cells in the Bolwig organ with GMR-hid.

(C) Thermal preferences of control (w1118), GMR-hid, and GMR-hid larvae carrying either the rh5G or rh6G mutations. n=6-7.

(D, E, F) Fraction of larvae in the 18°C zone of an 18°—28°C thermal gradient after expressing UAS-kir using the indicated GAL4 lines. The control larvae harbored the UAS-kir (UAS-kir/+) transgene only. n=4-5.

(G) Distribution of the indicated trpA1 mutants in the six indicated temperature zones. n=3-4.

See also Figure S3.