Larvae of the indicated genotypes were assayed on 18°—28°C thermal gradients.
(A) Percentages of larvae expressing UAS-rh5RNAi (orange) or UAS-rh6RNAi (blue) transgenes under control of the indicated GAL4 lines in the 18°C zone. n=5-7.
(B) Percentages of larvae expressing dicer2;UAS-trpA1RNAi under control of the indicated GAL4 lines in the 18°C zone. n=4-5.
(C) Testing for rescue of the rh61 mutant phenotype by expressing UAS-rh6 under control of trpA1-ABG4 (ABG4/+), trpA1-CDG4 (CDG4/+) or both GAL4s (ABG4/CDG4). n=5-6.
(D) Assaying thermotactic behavior of plc mutants (norpAP24 and plc21cP319) and the Gqα mutant (Gα49B1). n=4-6.
(E) Thermal distribution of larvae expressing UAS-Gα49BRNAi under control of the indicated GAL4 lines. n=4-6.
See also Figure S5.