Table S2.
Primer | Sequence |
BmMDH3-sSH3lig_F | gatataCATATGAAAAATACGCAGTCCGCATTCTAC |
BmMDH3-sSH3lig_R1 | TCCTCCAGACCCTCCGCCGCCCATCGCGTTTTTGATGATCTGGAT |
BmMDH3-sSH3lig_R2 | CGGAAGAGCTGGGGGAGGGGAGCCCCCACCACCTGAACCGCCTCCTCCAGACCCTCCGCC |
BmMDH3-sSH3lig_R3 | accagaCTCGAGTTACAAGTCCTCTTCAGAAATAAGCTTTTGTTCGGATCCCCTTCGTCTTTTTGGCGGAAGAGCTGGGGGAGG |
Ptac_his6-HPS_F | gatataCATATGGGCAGCCATCATCATCATCATCACAGCGGCAAGCTCCAAGTCGCCATC |
Ptac_his6-HPS_R | gctgcgACTAGTTTATTACAAGTCCTCTTCAGAAATAAGCTTTTG |
Ptac_his6-PHI_F | gatataCATATGGGCAGCCATCATCATCATCATCACGGCAGCACGCAAGCCGCAGAAGCC |
Ptac_his6-PHI_F | gctgcgACTAGTTTATTACTCGAGGTTGGCGTGGCG |
XbaI_SH3-HPS_F1 | GATATAATGGGCAGCCATCATCATCATCATCACAGCGGCATGGCAGAGTATGTGCGGGCC |
XbaI_SH3-HPS_F2 | ttccccTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATAATGGGCAGCCATCATCATCATCATCAC |
NdeI_SH3-HPS_R | gagcttCATATGACCAGAACCACCACCAGAACCACCATACTTCTCCACGTAAGGGACAGGAATCAT |
SLIC-ELPs1-F1 | cccgccgggcggcagcCCgcGGACTAGTCCTCCCCCAGCTCTTCCGCCAAAAAGACGAAGG |
SLIC-ELPs1-R1 | cagtggtggtggtggtggtGCTCGAGCCTTCGTCTTTTTGGCGGAAGAGCTGGGGGAGGACTAGTCC |
SacII-SH3 FP | atatCCGCGGATGGCAGAGTATGTGCGG |
XhoI-SH3 RP | atatCTCGAGATACTTCTCCACGTAAGGGAC |
Shown are the primers used to clone all of the enzymes in this study as well as the SH3 domain and the sSH3ligand (as a fusion to MDH). Underlined sequences represent restriction enzyme cut sites.