Skip to main content
. 2016 Oct 24;113(45):12691–12696. doi: 10.1073/pnas.1601797113

Table S2.

Primers used in this study

Primer Sequence
BmMDH3-sSH3lig_F gatataCATATGAAAAATACGCAGTCCGCATTCTAC
BmMDH3-sSH3lig_R1 TCCTCCAGACCCTCCGCCGCCCATCGCGTTTTTGATGATCTGGAT
BmMDH3-sSH3lig_R2 CGGAAGAGCTGGGGGAGGGGAGCCCCCACCACCTGAACCGCCTCCTCCAGACCCTCCGCC
BmMDH3-sSH3lig_R3 accagaCTCGAGTTACAAGTCCTCTTCAGAAATAAGCTTTTGTTCGGATCCCCTTCGTCTTTTTGGCGGAAGAGCTGGGGGAGG
Ptac_his6-HPS_F gatataCATATGGGCAGCCATCATCATCATCATCACAGCGGCAAGCTCCAAGTCGCCATC
Ptac_his6-HPS_R gctgcgACTAGTTTATTACAAGTCCTCTTCAGAAATAAGCTTTTG
Ptac_his6-PHI_F gatataCATATGGGCAGCCATCATCATCATCATCACGGCAGCACGCAAGCCGCAGAAGCC
Ptac_his6-PHI_F gctgcgACTAGTTTATTACTCGAGGTTGGCGTGGCG
XbaI_SH3-HPS_F1 GATATAATGGGCAGCCATCATCATCATCATCACAGCGGCATGGCAGAGTATGTGCGGGCC
XbaI_SH3-HPS_F2 ttccccTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATAATGGGCAGCCATCATCATCATCATCAC
NdeI_SH3-HPS_R gagcttCATATGACCAGAACCACCACCAGAACCACCATACTTCTCCACGTAAGGGACAGGAATCAT
SLIC-ELPs1-F1 cccgccgggcggcagcCCgcGGACTAGTCCTCCCCCAGCTCTTCCGCCAAAAAGACGAAGG
SLIC-ELPs1-R1 cagtggtggtggtggtggtGCTCGAGCCTTCGTCTTTTTGGCGGAAGAGCTGGGGGAGGACTAGTCC
SacII-SH3 FP atatCCGCGGATGGCAGAGTATGTGCGG
XhoI-SH3 RP atatCTCGAGATACTTCTCCACGTAAGGGAC

Shown are the primers used to clone all of the enzymes in this study as well as the SH3 domain and the sSH3ligand (as a fusion to MDH). Underlined sequences represent restriction enzyme cut sites.