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. 2016 Oct 25;113(45):E7097–E7105. doi: 10.1073/pnas.1606351113

Fig. 5.

Fig. 5.

The isolation-induced increase in stable actin interferes with the synaptic delivery of AMPARs. (A) Representative images showing SEP-GluA1 and tdTomato-actin fluorescence on spines before and after TEA treatment in the mPFC of socially isolated or nonisolated rats. (B) Quantification of changes in the SEP-GluA1 spine/ dendrite ratio after TEA treatment (n = 38 spines nonisolated and 45 spines isolated). (C) Two representative images. The first image shows a low unrecoverable actin fraction and a chemical LTP-induced increase in surface SEP-GluA1 (Example spine 1); the second image shows high unrecoverable actin and no LTP-induced increase in surface SEP-GluA1 (Example spine 2). The scatter plot shows the change in SEP-GluA1 intensity (presented as spine/ dendrite ratio) after TEA treatment versus the unrecoverable fraction of tdTomato-actin at individual spines. (n = 35 spines isolated: r = 0.371, P < 0.05; n = 26 spines nonisolated: r = −0.41, P > 0.5). Arrowheads indicate dendritic spines. *P < 0.05 (unpaired Student’s t test). Error bars represent SEM. (Scale bars: 1 μm.)