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. 2016 Oct 25;113(45):E7097–E7105. doi: 10.1073/pnas.1606351113

Fig. S7.

Fig. S7.

(A) FRAP analysis of spines of GFP-actin– and tdTomato-expressing neurons in the mPFC. Although the fluorescence levels of tdTomato are completely recovered, the fluorescence recovery of GFP-actin is partial, and there will be an unrecoverable fraction (n = 20 spines). (B) Social isolation does not affect spine size. Representative images showing tdTomato fluorescence on spines in the mPFC of socially isolated or nonisolated rats. There was no significant difference in the tdTomato spine/dendrite ratio between nonisolated and isolated rats (n = 33 spines nonisolated and 39 spines isolated). Representative images shown in the figures were unsharp mask-filtered. Arrowheads indicate dendritic spines. Error bars represent SEM. n.s., not statistically significant (unpaired Student’s t test). (Scale bars: 1 μm.)