Skip to main content
. 2016 Oct 25;113(45):E7097–E7105. doi: 10.1073/pnas.1606351113

Fig. S9.

Fig. S9.

Constitutive-active ADF/cofilin is moderately overexpressed in electroporated animals. (A) The level of endogenous cofilin is similar between RFP-S3A transfected (n = 35 neurons) and untransfected (n = 35 neurons) neurons. Arrowheads indicate S3A transfected neurons. (Scale bar: 20 μm.) (B) (Left) Representative images showing GFP fluorescence on spines in the mPFC of socially isolated or nonisolated rats. Arrowheads indicate mushroom-shaped spines; arrows indicate stubby spines. (Scale bars: 2 μm.) (Right) High-magnification images of dendritic spine enclosed with rectangles. Representative images shown in the figures were Gaussian- and unsharp mask-filtered. (Scale bars: 1 μm.) (C) There was no significant difference of spine shapes (n = 25 dendrites, vector; n = 26 dendrites, S3A). (D) There was no significant difference in the GFP spine/dendrite ratio between nonisolated and isolated rats (n = 61 spines, vector; n = 61 spines, S3A). (E and F) Sholl analysis of basal dendrites in the mPFC layer 2/3 pyramidal cell based on distance from the cell body. (E) Mean length of basal dendrite branches of PrL regions (n = 10 neurons, vector; n = 11 neurons, S3A). (F) Mean number of intersections of basal dendrite branches with consecutive 5-μm-spaced concentric spheres of PrL regions (n = 10 neurons, vector; n = 11 neurons, S3A) [repeated measures ANOVA F(1, 730) = 0.506]. Error bars represent SEM. n.s., not statistically significant (A, C, D, and E, unpaired Student’s t test; F, repeated measures ANOVA).