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Use of CAPS marker to follow the inheritance of SSIIIa genes. (A) Positions of primers used to amplify a 282-bp fragment spanning the mutation site in b10. (B) Genotyping of SSIIIa alleles using the SSIIIa CAPS marker. M represents DNA size markers. Lane 1, DNA from wild-type plants (R7954); lanes 2, 3, and 4 represent homozygous SSIIIaSSIIIa, heterozygous SSIIIassIIIa, and homozygous ssIIIassIIIa plants, respectively.
