Fig. S1.

Presence of Mili transcript in the adult brain tissues. Visualization of piwil2 (Mili), piwil1 (Miwi), and piwil4 (Miwi2) transcript alignment from poly(A) selected from HP and PFC and from ENCODE long RNA-Seq contigs, from cerebellum, cortex, and frontal lobe of 8-wk-old mice. Each gene with respective intron-exon boundaries was delineated on top of each panel (blue), with subsequent demonstration of the transcripts from our laboratory-prepared hippocampal and PFC cDNA libraries (first two tracks from top) and RNA-Seq contigs from three brain (cerebellum, cortex, and frontal lobe) and two nonbrain (lung and spleen) libraries (last five tracks) from ENCODE. Mili transcript alignment was reproduced for a side-by-side comparison with Miwi and Miwi2 transcript alignment (Fig. 1A). Note, consistency observed in piwi expression patterns between our laboratory-prepared and ENCODE brain libraries. Further note depletion of Piwil2 (Mili) in nonbrain libraries.