. 2016 Oct 10;113(45):E7106–E7115. doi: 10.1073/pnas.1608519113

Table S5.

α7-AChR–β-GluCl chimera reversal potentials and permeability-coefficient ratios

α7-AChR–β-GluCl filter motif^*	Other mutations	Reversal potential, mean ± SE, mV^†	P_K⁺/P_Cl^–, mean ± SE^‡	P_Cl^–/P_K⁺, mean ± SE^‡	n^§
IDLHSTAGR	—	+47.8 ± 1.0	0.003	410	6
IDLHSTAGG	—	+23.9 ± 0.7	0.17 ± 0.01	5.8 ± 0.3	5
IDLHSTAGN	—	−9.1 ± 1.4	1.1 ± 0.1	0.89 ± 0.07	3
IDLHSTAGN	D96N + E97Q (ECD)^¶	+11.9 ± 2.1	0.37 ± 0.05	2.8 ± 0.3	3

Mutant residues are given in bold.

^{^†}

The equilibrium (Nernst) potentials at 22 °C, calculated using ion activities, were –50.6 mV for K⁺, and +46.2 mV for Cl^–.

^{^‡}

Means and SEs were calculated as described in detail in SI Materials and Methods. Therefore, the mean values of the permeability ratios in this table (columns 4 and 5) are different from the permeability ratios that could be calculated by applying the GHK equation to the mean reversal potentials (column 3).

^{^§}

Number of independent whole-cell recordings.

^{^¶}

Extracellular domain.