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. 2016 Oct 24;113(45):E7087–E7096. doi: 10.1073/pnas.1609111113

Fig. S5.

Fig. S5.

Validation of a selection of genes as identified in the FoxO6 knockdown genome-wide transcriptome analysis. In an independent set of experiments, cortices were in utero electroporated with siFoxO6-1 and cotransfected with a GFP expression vector, followed by FACsorting and RNA isolation. Genes significantly regulated according to the transcriptome data were investigated by qPCR. Significant down-regulation of FoxO6, Plxna4, Nfia, and Pik3ca was confirmed. Significant up-regulation of Xlr3c was also confirmed. Lmx1a was used as an unchanged control. The error bars show the SD. Two-tailed Student’s t test; *P < 0.05.