Fig. 2.

Shibire is not required for Wg endocytosis. (A–F) Confocal sections of wing discs from shi^ts1 (A, B, and D) or w¹¹¹⁸ (C) incubated at the permissive temperature of 23 °C (A and C) or at the restrictive temperature of 31–32 °C (B and D) showing 5- to 8-min pulsed endosomes of Dextran (A and B; red in A″ and B″), DFz2 (A′ and B′; green in A″ and B″), or Wg (C and D). E and F show quantification of the intensity of DFz2 and Wg endosomes, respectively, between control and shi^ts1 wing discs. Robust endocytic uptake of fluid and mBSA is observed in wing discs of w¹¹¹⁸ (32 °C) and shi^ts1 (room temperature-permissive) and both serve as controls for Dynamin function. Error bars indicate the SE calculated from three to six wing discs. P < 10⁻²⁵ in E. A″ and B″ indicate YZ projection of Control and shi^ts1 wing discs, respectively, along the planes indicated. Red and green arrows in A″ and B″ indicate the plane of the representative images. Boxed regions are magnified (∼2.7×) and shown below the respective images. The image in C has been rotated; all images background-subtracted and median-filtered with intensities appropriately scaled for representation. Although DFz2 endocytosis is affected in shi^ts1 wing discs shifted to restrictive temperature, both Dex and Wg endocytosis remain unaffected. (Scale bars, 50 μm.)