Fig. 4.

Ligand-based glycoprotein receptor capture identifies laminin subunits α4 and γ1 as NP41 binding partner candidates. (Right) Structure of NP41–FAM–HB conjugate. Fluorescein allows for visualization of the peptide, pyridylhydrazine cross-links the peptide to glycans after tissue is oxidized with sodium periodate, and biotin is used to isolate the peptide target from nerve homogenates. (Left) Glycoprotein receptor capture with NP41--FAM--HB versus all d-amino acid control conjugate identifies formerly glycosylated peptides laminin-γ1 (Lamc1) and -α4 (Lama4) peptides.