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. 2016 Oct 20;113(45):12774–12779. doi: 10.1073/pnas.1611642113

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Fig. S1. — Photooxidation of MB–biotin results in tryptophan oxidation of streptavidin. (A) Primary sequence of streptavidin with observed tryptic peptides underlined; tryptophans are highlighted and modified listed with mass identified by the SEQUEST algorithm. (B) Percent of peptides containing +4 kDa tryptophan mass change (kynurenine) increases with photooxidation. The area of the modified peak is plotted as a percentage of area of unmodified peak. Peak conversion occurred after 30 s of light exposure. (C) Percent of peptides containing +16 kDa tryptophan mass change increases with photooxidation. Peak conversion occurred after 3 s of light exposure.