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. 2016 Oct 26;113(45):12838–12843. doi: 10.1073/pnas.1612746113

Fig. 1.

Fig. 1.

Quantification of VGSC transcripts in heart RNA by quantitative RT-PCR. (A) Representative traces show that amplification of the Scn5a transcript requires fewer PCR cycles than amplification of the Scn8a transcript. (B) Data corrected for amplification of the internal control, Tbp. Transcript abundance does not differ between mutant and WT mice. The abundance of transcripts of three TTX-S channels Scn1a, Scn3a, and Scn8a is comparable. Together, they are ∼1% of the total VGSCs in heart. Each symbol represents RNA from one animal. D/+, Scn8aN1768D/+ (n = 3); +/+, Scn8a+/+ (n = 3).