Fig. 5.

Translated ORFs identified within annotated ncRNAs. (A–C) RNA-seq and P sites in ribosome footprints in root for three ORFs identified within annotated ncRNAs. The predicted CDS and 5′-UTR are depicted as black and gray boxes, respectively. The 3′-UTR is represented by a white arrow. Data presentation is the same as described in the legend of Fig. 3. (D) A schematic diagram of HA-tagged constructs and Western blot analysis of proteins produced by the three annotated ncRNAs in A–C. Total protein in root of control plants (Col-0) and transgenic plants expressing individual HA-tagged proteins was isolated and analyzed with either anti-HA antibody or anti-UGPase antibody as a loading control.