Fig. S3.
Proinflammatory mediator gene expression in NT-stimulated human microglia pretreated with control scrambled (Sc) and NTR3/sortilin siRNA. Control and siRNA-transfected microglia-SV40 (2 × 106 cells) were stimulated with NT (10 nM) for 24 h, and the culture medium was concentrated to measure release of IL-1β (A), CXCL8 (B), CCL2 (C), and CCL5 (D) by qRT-PCR. All conditions were performed in triplicate for each dataset and were repeated three times (n = 3). Results were normalized against the endogenous GAPDH and expressed relative to the mean of the control for the gene of interest, with significance of comparisons denoted by **P < 0.001 or ***P < 0.0001.