Fig. S6.

Microglia proinflammatory mediator gene expression in response to LPS is attenuated by the PI3K/mTOR inhibitors and the flavonoids Lut and Methlut. HM-SV40 (2.5 x10⁵ cells) were pretreated with the dual PI3K/mTOR (PF, 0.5 μM), the mTOR (Rap and KU, 0.1 μM) inhibitors, and the flavonoids (Lut and Methlut, 0.1 μM) for 2 h, and then stimulated with NT (10 nM) for 12 h in serum-free media to determine changes in gene levels of IL-1β (A), CXCL8 (B), CCL2 (C), and CCL5 (D) by qRT-PCR. All inhibitors were dissolved in water or DMSO with a final concentration <0.1%. All conditions were performed in triplicate for each dataset and were repeated three times (n = 3). Results were normalized against the endogenous gene GAPDH and are expressed relative to the mean of the gene of interest. Significance of comparisons was determined for stimulated cells and for those cells with inhibitors/flavonoids, as denoted by the horizontal lines (P < 0.0001), and also among all of the inhibitors/flavonoids treatments shown by the horizontal brackets and by corresponding *P < 0.05, **P < 0.001, and ***P < 0.0001.