Fig. 4.

Solution studies of CC domains with extended sequences of Sr33 and MLA10. Molecular mass calculations from SEC-MALS analysis for Sr33^6-120, Sr33^6-144, and Sr33^6-160 (A) and MLA10^5-120 and MLA10^5-144 (B). Solid gray, dark gray, and black lines represent the refractive index for the three proteins, respectively, when eluted from an in-line Superdex 200 5/150 GL column, normalized to the height of the major peak for clarity. Dotted lines indicate the predicted molecular masses of both monomeric and dimeric species, and colored lines show the experimental molecular mass distributions as determined by MALS (values are shown in SI Appendix, Table S5).