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. 2016 Oct 24;113(45):E7069–E7076. doi: 10.1073/pnas.1607849113

Fig. 4.

Fig. 4.

miR-515 family members are up-regulated in placentas from PE vs. gestation-matched normotensive women, whereas expression of miR-515-5p targets is significantly decreased. (A) RNA isolated from term placentas of PE (n = 18) and gestation-matched normotensive (CTR) women (n = 18) was analyzed for expression of several miR-515 family members using TaqMan based qRT-PCR. RNA from term placentas of PE and gestation-matched normotensive women (n = 18 per group) was analyzed for expression of CYP19A1, FZD5, and GCM1 mRNA (B) and protein (C). Relative expression of aromatase, FZD5 and GCM1 proteins was assessed by immunoblotting of normotensive (n = 18) and PE groups (n = 18). The immunoreactive bands were scanned and normalized to β-actin scanned on the same immunoblot. Values are presented as mean ± SEM. *Significantly different from CTR, P < 0.05.