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Sequences within the 3′UTRs of CYP19A1, GCM1, and FZD5 containing one or more putative binding sites for miR-515-5p that were subcloned into the psiCHECK-2 vector to create wild-type or mutated (m) luciferase reporter plasmids. Shown are the positions and sequences of the WT and mutated (m) putative miR-515-5p response elements within the 3′UTRs of CYP19A1, GCM1, and FZD5. The bases within the 3′UTR of each mRNA that were mutated are indicated in red.
