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. 2016 Oct 24;113(45):E7069–E7076. doi: 10.1073/pnas.1607849113

Fig. S3.

Fig. S3.

c-Myc does not bind specifically to E-boxes 1–3 upstream of miR-515-1 or to E-box 2 upstream of miR-515-2. Binding of c-Myc to genomic regions surrounding E-boxes 1 and 2 and E-box 3 upstream of the miR-515-1 precursor (A) and to E-box 2 upstream of the miR-515-2 precursor (B) was evaluated in freshly isolated human CytT before (0 h) and after 40 h of culture using ChIP-qPCR. Before culture, the cells were infected with recombinant adenovirus expressing β-Gal or c-Myc. In contrast to what was observed for miR-515-1 E-box 4 and miR-51-2 E-box 1 (Fig. 5), binding of c-Myc to E-boxes 1–2 and 3 upstream the miR-515-1 gene (A) and to E-box 2 upstream the miR-515-2 gene (B) was not significantly different from the IgG control at each time point and treatment.