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. 2016 Oct 24;113(45):12715–12720. doi: 10.1073/pnas.1609227113

Fig. 3.

Fig. 3.

NAD+ salvage pathway determines metabolic transitions in the cell nucleus. (A) Schematic representation of the NAD+ metabolic salvage pathway. NMNAT1–3, nicotinamide mononucleotide adenylyltransferase 1–3. (B–F) Intensity and FLIM (lifetime) images from (B, C, and E) WT MEFs and (D and F) Sirt1−/− MEFs nontreated or treated with (B) NAD+ (2 mM), (C and D) NAM (10 mM, 16 h), and (E and F) FK866 (10 nM). Histograms in Right show comparative analyses in the percentage change on the fraction of free NADH. Data were normalized for nontreated MEFs = 0. Means ± SEM of three independent experiments are presented. ns, not significant by two-tailed MWU; nt, nontreated. *P < 0.05 by two-tailed MWU; **P < 0.01 by two-tailed MWU.