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. 2016 Oct 24;113(45):12815–12819. doi: 10.1073/pnas.1613308113

Fig. 1.

Fig. 1.

Multiple cbb3 isoforms in P. aeruginosa. (A) Sixteen isoforms of cbb3 could be produced via combinations of the isosubunits encoded by four cco gene clusters. cbb3-1 and cbb3-2 are shown in boxes. (B) Cytochrome c oxidase activity of the isoforms visualized in blue color by the Nadi assay. WT and Δcbb3 indicate PAO1 and the ccoN1O1Q1P1 ccoN2O2Q2P2 double-mutant MCb12, respectively. Triple-digit numbers indicate the combination of the CcoN, CcoO, and CcoP isosubunits. For example, 111, 212, 321, and 422 indicate CcoN1O1P1, CcoN2O1P2, CcoN3O2P1, and CcoN4O2P2, respectively. The isoforms were expressed in strain STO7 with the expression plasmids shown in Fig. S1.