Table S2.
Bacterial strains and plasmids used in this study
| Strains or plasmids | Relevant characteristic(s) | Source or reference |
| P. aeruginosa strains | ||
| PAO1 | PAO1 of the University of Washington (MPAO1) used as a wild-type | (27) |
| PW4739 | ΔhcnB (PA2194) from mutant library at Univ. of Washington | (27) |
| MCb12 | ΔccoN1O1Q1P1, ΔccoN2O2Q2P2 of MPAO1 | This study |
| MSccoN3 | ΔccoN3 of MPAO1 | This study |
| MSccoN4 | ΔccoN4 of MPAO1 | This study |
| PTO5 | ΔcyoABCDE, ΔcioAB, ΔcoxBA-coxC, ΔccoN1O1Q1P1, ΔccoN2O2Q2P2 of PAO1ut | (10) |
| STO7 | ΔccoN3, ΔccoN4 of PTO5 | This study |
| Plasmids | ||
| pMMB67EH | IncQ, expression vector; Apr, Cbr | (28) |
| pEX18Ap | Gene replacement vector; Apr, Cbr, oriT+, sacB+ | (29) |
| pUC18-mini-Tn7T-Gm-lacZ | mini-Tn7T, lacZ transcriptional fusion vector; Apr, Gmr | (30) |
| pMMB-N1O1Q1P1 | ccoN1O1Q1P1 on pMMB67EH | This study |
| pMMB-N1O1Q1P2 | ccoN1O1Q1P2 on pMMB67EH | This study |
| pMMB-N1O2Q2P1 | ccoN1O2Q2P1 on pMMB67EH | This study |
| pMMB-N1O2Q2P2 | ccoN1O2Q2P2 on pMMB67EH | This study |
| pMMB-N2O1Q1P1 | ccoN2O1Q1P1 on pMMB67EH | This study |
| pMMB-N2O1Q1P2 | ccoN2O1Q1P2 on pMMB67EH | This study |
| pMMB-N2O2Q2P1 | ccoN2O2Q2P1 on pMMB67EH | This study |
| pMMB-N2O2Q2P2 | ccoN2O2Q2P2 on pMMB67EH | This study |
| pMMB-N3Q3O1Q1P1 | ccoN3Q3O1Q1P1 on pMMB67EH | This study |
| pMMB-N3Q3O1Q1P2 | ccoN3Q3O1Q1P2 on pMMB67EH | This study |
| pMMB-N3Q3O2Q2P1 | ccoN3Q3O2Q2P1 on pMMB67EH | This study |
| pMMB-N3Q3O2Q2P2 | ccoN3Q3O2Q2P2 on pMMB67EH | This study |
| pMMB-N4Q4O1Q1P1 | ccoN4Q4O1Q1P1 on pMMB67EH | This study |
| pMMB-N4Q4O1Q1P2 | ccoN4Q4O1Q1P2 on pMMB67EH | This study |
| pMMB-N4Q4O2Q2P1 | ccoN4Q4O2Q2P1 on pMMB67EH | This study |
| pMMB-N4Q4O2Q2P2 | ccoN4Q4O2Q2P2 on pMMB67EH | This study |
| pEX-ΔccoN3 | Plasmid for ccoN3 mutation, a derivative of pEX18Ap | This study |
| pEX-ΔccoN4 | Plasmid for ccoN4 mutation, a derivative of pEX18Ap | This study |
| pUC-ccoN3-lacZ | mini-Tn7T; ccoN3::lacZ transcriptional fusion | This study |
| pUC-ccoN4-lacZ | mini-Tn7T; ccoN4::lacZ transcriptional fusion | This study |
Apr, ampicillin resistant; Cbr, carbenicillin resistant; Gmr, gentamicin resistant.