Table 3.

Top predicted destabilizing mutants based on the E2 core structure

Mutant	Score*	Average A-D†
Y611A	6.4	0.8
W616A	4.8	84.2
R614A	4.7	0.9
F550A	4.6	25.8
F509A	4.3	0.7
Y507A	3.8	14.1
W554A	3.8	0.4
W549A	3.8	37.8
W437A	3.8	73.0
F537A	3.5	5.2

Mutants in bold denote measured destabilizing mutants based on reactivity to conformation-dependent antibodies for antigenic domains A-D. Mutants in italics exhibit major disparity between antibody-inferred E2 stability and impact on stability predicted from the E2 core structure (18).

^*Rosetta score (35) for predicted energetic impact of mutant on E2.

^†Average percent binding versus wild-type E2 for conformation-dependent E2 antibodies.