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. 2015 Dec 16;55(12):1940–1951. doi: 10.1002/mc.22441

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© 2015 The Authors. Molecular Carcinogenesis Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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qPCR gene expression data for Caco‐2 cells following reconstitution of a functional TP53 locus, using CpG variable alleles (WT construct; CpG‐enriched construct; CpG‐depleted construct) with and without in vitro methylation prior to integration, for fold change of full‐length P1 TP53 and P2 mRNAs. A: Methylated. B: Unmethylated. OFF, construct integrated. ON, construct integrated and activated. ON XR, active construct additionally X‐irradiated. Data are normalized to β‐actin. Fold change is relative to the original cell source (OFF).