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. 2016 Apr 14;231(11):2517–2528. doi: 10.1002/jcp.25388

Figure 1.

Figure 1

Both 25(OH)D3 and 1,25(OH)2D3 attenuated C2C12 myoblast proliferation. Micrographs of C2C12 myoblasts cultured for 4 days in growth medium (A), in growth medium supplemented with 1000 nmol/L 25(OH)D3 (B), or 100 nmol/L 1,25(OH)2D3 (C). Myoblast proliferation was quantified at day 1 and 4 (D). Scale bar indicates 100 µm. Data were analyzed using a two‐way ANOVA followed by Bonferroni's post hoc comparisons test. Values are mean ± SEM (n = 20). ***P < 0.001; ### P < 0.001 (# between time period, * between vitamin D3 concentrations).