Table 1.
Comparison basal extracellular concentration by the microfabricated probes, conventional probes with previous reports. The in vivo recovery for the microfabricated probes is estimated by finding the ratio between [μFab] and [MD]. All measurements are from sampling of the striatum of rats. Values given as mean ± SEM (n = 5 animals). In vivo recovery of μFab probe estimated by dividing μFab concentration by MD concentration, where recovery was estimated at 100%.
| Analyte | Previously-reported concentration, μM (Anesthesia, Quantification method, Ref #) | Dialysate concentration, μM | Estimated in vivo recovery, % | |
|---|---|---|---|---|
| [MD] | [ufab] | |||
| ACh | 0.01 ± 0.003 (Ketamine, 20% in vitro recovery, 34) | 0.032 ± 0.0045 | 0.0045 ± 0.00046 | 14 ± 0.4 |
| Choline | 11 ± 0.93 | 0.54 ± 0.022 | 5 ± 0.2 | |
| DOPAC | 17.4 ± 2.6 (Awake, No-net-flux, 37) | 8.9 ± 1.4 | 0.74 ± 0.084 | 8 ± 0.8 |
| DOPA | 0.15 ± 0.017 | 0.0056 ± 0.00041 | 4 ± 0.3 | |
| Dopamine | 0.010 ± 0.0017 (Awake, No-net-flux, 37) | 0.0067 ± 0.00062 | 0.00032 ± 0.000046 | 5 ± 0.3 |
| Glucose | 350 ± 20 (Awake, No-net-flux, 40) | 1431 ± 82 | 56 ± 3.3 | 4 ± 0.2 |
| GABA | 0.12 ± 0.05 (Urethane, 20% in vitro recovery, 38) | 0.78 ± 0.34 | n.d. | - |
| Glutamine | 385 ± 16 (Awake, No-net-flux, 42) | 472 ± 24 | 18 ± 2.2 | 4 ± 0.3 |
| Histamine | 0.039 ± 0.016 | n.d. | - | |
| 5-HIAA | 3.5 ± 0.1 (Awake, Low-flow-rate, 37) | 1.2 ± 0.13 | 0.095 ± 0.016 | 8 ± 1.2 |
| HVA | 17 ± 1.1 (Awake, Low-flow-rate, 37) | 8.3 ± 1.1 | 0.81 ± 0.097 | 10 ± 0.9 |
| 3-MT | 0.0069 ± 0.00046 | n.d. | - | |
| Phe | 2 ± 0.2 (Chloral Hydrate, Low-flow-rate, 39) | 7.5 ± 0.49 | 0.22 ± 0.012 | 3 ± 0.1 |
| Serine | 7 ± 0.5 (Awake, Corrected for in vitro recovery, 41) | 36 ± 2.3 | 1.8 ± 0.12 | 5 ± 0.2 |
| 5-HT | 0.019 ± 0.0042 | n.d. | - | |
| Taurine | 25 ± 5.1 (Halothane, No-net-flux, 43) | 60 ± 4.9 | 2.8 ± 0.21 | 4 ± 0.1 |
| Tyrosine | 3.3 ± 0.9 (Chloral Hydrate, Low-flow-rate, 39) | 6.7 ± 0.33 | 0.22 ± 0.0011 | 3 ± 0.1 |