Table 1. Preliminary selection of maximum tolerable dose of PH for experimental animals.

Animal groups	Dose (mg/kg-1 bw)	Dose interval (h)	Total number of doses (n)	Total dose (mg/kg-1 bw)	Total duration of treatment (h)	Physical signs	Tolerance by animals
1	5	12	2	10	24	No physical change	Survived
	5	12	4	20	48	Slight appearance of yellow color of skin and eyes	Survived
	5	12	8	40	96	Slight change in skin and eye color	Survived
2	5	24	2	10	48	No physical change	Survived
	5	24	4	20	96	Slight appearance of yellow color of skin and eyes	Survived
	5	24	8	40	192	Slight appearance of yellow color of skin and eyes	Survived
3	5	48	2	10	96	No physical change	Survived
	5	48	4	20	192	No physical change	Survived
	5	48	8	40	382	Slight appearance of yellow color of skin and eyes	Survived
4	25	12	2	50	24	Appearance of deep yellow color of skin and eyes	Survived
	25	12	4	100	48	Anemic and lethargic response, gray color of skin and eyes due to excess hemolysis	Survived
	25	12	8	200	96	Swear anemic response, dark gray color of skin and eyes due to almost complete hemolysis	Died after 8th dose
5	25	24	2	50	48	Appearance of deep yellow color of skin and eyes	Survived
	25	24	4	100	96	Appearance of deep yellow color of skin and eyes	Survived
	25	24	8	200	192	Anemic and lethargic response, gray color of skin and eyes due to excess hemolysis	Survived
6	25	48	2	50	96	No physical change	Survived
	25	48	4	100	192	Appearance of deep yellow color of skin and eyes	Survived
	25	48	8	200	382	Anemic and lethargic response, gray color of skin and eyes due to excess hemolysis	Survived
7	50	12	2	100	24	Anemic and lethargic response, gray color of skin and eyes due to excess hemolysis	Survived
	50	12	4	200	48	Swear anemic response, dark gray color of skin and eyes due to almost complete hemolysis	Died after 4th dose
	50	12	8	400	Not treated
8	50	24	2	100	48	Anemic and lethargic response, gray color of skin and eyes due to excess hemolysis	Survived
	50	24	4	200	96	Anemic and lethargic response, gray color of skin and eyes due to excess hemolysis	Survived
	50	24	8	400	192	Swear anemic response, dark gray color of skin and eyes due to almost complete hemolysis	Died after 7th dose
9	50	48	2	100	96	Appearance of deep yellow color of skin and eyes	Survived
	50	48	4	200	192	Anemic and lethargic response, gray color of skin and eyes due to excess hemolysis	Survived
	50	48	8	400	382	Swear anemic response, dark gray color of skin and eyes due to almost complete hemolysis	Died after 8th dose