FIG. 9.

PBP1 and membrane binding sites in the SAXS model of GpsB. The atomic coordinates of N-GpsB dimers and C-GpsB trimers, represented as molecular surfaces (green and magenta, respectively), have been manually positioned in the averaged ab initio molecular envelope of the LmGpsB hexamer, shown as a gray mesh. Surface exposed residues critical for the interaction of LmGpsB with PBP1¹¹ are colored red (D33, D37, I40). Residues critical for association of LmGpsB with the cell membrane¹¹ are colored blue (L24, R25). The molecular envelope represented here is the average of 15 independently generated ab initio dummy atom models.