Table 1.
Strains and Plasmids Used in This Study
| Name | Relevant characteristics | Sourcea/reference |
|---|---|---|
| Plasmids | ||
| pSH254 | Phelp-lacO-gpsB lacI neo | 11 |
| pJR133 | Phelp-lacO-gpsB T88A lacI neo | This work |
| pJR134 | Phelp-lacO-gpsB T88D lacI neo | This work |
| pSH433 | Phelp-lacO-gpsB F91A lacI neo | This work |
| pSH434 | Phelp-lacO-gpsB L94A lacI neo | This work |
| pSH435 | Phelp-lacO-gpsB F105A lacI neo | This work |
| Listeria monocytogenes strains | ||
| EGD-e | wild-type, serovar 1/2a strain | 54 |
| LMJR19 | ΔgpsB (lmo1888) | 11 |
| LMS56 | ΔgpsB attB::Phelp-lacO-gpsB lacI neo | 11 |
| LMJR161 | ΔgpsB attB::Phelp-lacO-gpsB T88A lacI neo | pJR133 → LMJR19 |
| LMJR162 | ΔgpsB attB::Phelp-lacO-gpsB T88D lacI neo | pJR134 → LMJR19 |
| LMS185 | ΔgpsB attB::Phelp-lacO-gpsB F91A lacI neo | pSH433 → LMJR19 |
| LMS186 | ΔgpsB attB::Phelp-lacO-gpsB L94A lacI neo | pSH434 → LMJR19 |
| LMS187 | ΔgpsB attB::Phelp-lacO-gpsB F105A lacI neo | pSH435 → LMJR19 |
a
The arrow (→) stands for a transformation event.