Table 1.
Melting transitions (Tm) and free energies of complexation (−ΔG°37) for the circular oligodeoxynucleotides 1 and 2 with their complements and for the analogous duplexes at pH 7.0. Estimated uncertainties in Tm are ±0.5 °C and in free energy, ±10%.
| Complex | Tm/°C | −ΔG°37/kcala |
|---|---|---|
| 5′-AAAAAAAAAAAA 3′-TTTTTTTTTTTT |
36.9 | 8.1 |
|
|
57.4 | 16.7 |
| 5′-AAGAAAGAAAAG 3′-TTCTTTCTTTTC |
44.7 | 9.8 |
|
|
61.8 | 16.5 |
1 cal = 4.184J.