Table 1.

Sequences of the primers used to amplify the msp-1 and msp-2 genes of P. falciparum isolates

Locus	Primer	Primer sequence
Primary PCR
msp-1	msp1-P1	5′-CACATGAAAGTTATCAAGAACTTGTC-3′
	msp1-P2	5′-GTACGTCTAATTCATTTGCAC-3′
msp-2	msp2-1	5′-ATGAAGGTAATTAAAACATTGTCTATTATA-3′
	msp2–4	5′-ATATGGCAAAAGATAAAACAAGTG-3′
Secondary PCR
msp-1	K1-K1	5′-GAAATTACTACAAAAGGTGCAAGTG-3′
	K1–K2	5′-AGATGAAGTATTTGAACGAGGTAAAGTG-3′
	MAD20-M1	5′-GAACAAGTCGAACAGCTGTTA-3′
	MAD20-M2	5′-TGAATTATCTGAAGGATTTGTACGTCTTGA-3′
	R033-R1	5′-GCAAATACTCAAGTTGTTGCAAAGC-3′
	R033-R2	5′-AGGATTTGCAGCACCTGGAGATCT-3′
msp-2	3D7-A1	5′-GCAGAAAGTAAGCCTTCTACTGGTGCT-3′
	3D7-A2	5′-GATTTGTTTCGGCATTATTATGA-3′
	FC27-B1	5′-GCAAATGAAGGTTCTAATACTAATAG-3′
	FC27-B2	5′-GCTTTGGGTCCTTCTTCAGTTGATTC-3′