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. 2016 Jun 28;24(10):1734–1744. doi: 10.1038/mt.2016.109

Figure 1.

Figure 1

Discoidin domain receptor 2 (DDR2) facilitates experimental lung fibrosis. (a–d) Ddr2 deficient mice were resistant to bleomycin-induced fibrosis in the lung. Wild-type or slie mutant mice were administered bleomycin and the lung tissues were harvested 4 weeks later. Representative images of H&E a and Masson's trichrome staining b are shown. Scale bars, 100 µm. The whole lung homogenates from three mice were used for hydroxyproline content assay and immunoblot analysis, respectively c. Immunofluorescent staining of myofibroblast marker α-smooth muscle actin (α-SMA) (red) was demonstrated in d. Right histogram shows the percentage of α-SMA staining area with regard to the total picture area (10 fields/group). **P < 0.01. (e–g) DDR2 knockdown prevents pulmonary fibrosis. One day before bleomycin challenge, C57BL/6 mice were instilled with small interfering RNA (siRNA) solutions and this treatment was repeated once a week until the lung tissues were collected on day 28 (e). The protein extracts from three mice were pooled and subjected to immunoblot (f). The images in g show H&E and Masson's trichrome staining, respectively. Scale bars, 100 µm. H&E, Hematoxylin and Eosin.