Skip to main content
. 2016 Aug 30;24(10):1726–1733. doi: 10.1038/mt.2016.151

Figure 3.

Figure 3

The effects of lncRNA-MSR on TMSB4 expression and ECM in chondrocytes. (a, b) The mRNA expression of TMSB4, COL2A1, ACAN, MMP13, and ADAMTS5 were determined after overexpression of lncRNA-MSR or siRNA knockdown of lncRNA-MSR. (c) The chondrocytes were transfected with p-lncMSR or si-MSR, and the protein expression of COL2A1 and MMP-13 was analyzed using Western blotting. GAPDH was used as loading control. (d, e) The chondrocytes were transfected with p-lncMSR or si-MSR, and the cells were fixed and stained with rhodamine phalloidin and Hoechst 33342. Bar = 100 µm. The results are shown as the mean ± SEM of at least three independent experiments (*P < 0.05, **P < 0.01 p-lncMSR versus control (treated with empty plasmid) and si-MSR versus the negative control (NC) siR-Ribo).