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. 2016 Aug 30;24(10):1726–1733. doi: 10.1038/mt.2016.151

Figure 4.

Figure 4

LncRNA-MSR is targeted by TMSB4-targeting miR-152. (a) TMSB4 and lncRNA-MSR 3'-UTRs contain a highly conserved domain. Targeted miRNA seed matches within the high homology region are conserved between TMSB4 and lncRNA-MSR. The miRNAs binding to TMSB4 and lncRNA-MSR are matched by solid lines. (b, c) The luciferase reporter analysis of either the wild-type or mutant TMSB4 and lncRNA-MSR 3'-UTR activity. The miRNAs were cotransfected with the wild-type or mutant vector, and scrambled miRNA was used as a negative control. (d) The primary chondrocytes were transfected with the miR-152 mimic or inhibitor. Scrambled miRNA was used as a negative control. The expression of lncRNA-MSR and TMSB4 was analyzed using qPCR. (e) The protein expression of TMSB4 was analyzed using western blotting with GAPDH as loading control. The results are shown as the mean ± SEM of at least three independent experiments (*P < 0.05, **P < 0.01).