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. Author manuscript; available in PMC: 2017 Nov 15.
Published in final edited form as: Immunity. 2016 Oct 25;45(5):1052–1065. doi: 10.1016/j.immuni.2016.10.005

Figure 4. Fas-Dependent cDC Regulation of the AM14 Response is AM14 B Cell Extrinsic.

Figure 4

(A) Experiment design. 8 × 106 Fas-deficient AM14.MRL.Faslpr B cells were transferred into either Fas-sufficient, cDC-deficient (ΔDC) MRL.Faslpr/+ recipients or into littermate controls (Cntrl) on day 0. The AM14 B cell response was activated with PL2-3 on days 0, 2 and 4. Some mice did not receive cell transfer or Ag (no Tfr). Spleens were harvested on day 6.

(B) Number of AM14 B cells (surface and intracellular 4-44+) per spleen.

(C) Number of AM14 plasmablasts (4-44+, CD22lo, CD44hi) per spleen.

(D, E, & F) ELISpot assays for the number of 4-44+, IgM+, IgG2a+, or IgG2b+ AFCs per spleen. Data were combined from 2 experiments to obtain 8-15 mice per group receiving PL2-3 and 2 no Tfr controls. Bars represent mean and SEM. ***p < 0.001 by Mann-Whitney U test.

See also Figure S7.