Figure 6. Regulation of AM14 Plasmablasts by cDCs Requires T Cells Expressing Fas.

(A) Experiment design. BALB/c mice were used to make chimeras with BM mixed from WT, Tcra^−/− or Fas^lpr mice. 3 × 10⁶ AM14.BALB/c B cells were transferred into the mice on day 0, and activated with PL2-3 on days 0, 2 and 4. Spleens were harvested on day 6.

(B, C, & D) ELISpot assays for the number of 4-44⁺, IgM⁺, IgG2a⁺, or IgG2b⁺ AFCs per spleen.

(E) Number of CD4⁺, TCRβ⁺ cells per spleen.

(F) Percent of CD4⁺, TCRβ⁺ cells that are activated (CD44^hi, CD62L^lo).

(G) Number of CD4⁺, TCRβ⁺, CD44^hi, CD62L^lo cells per spleen.

Data were combined from 2 experiments to obtain 6-12 mice per group receiving PL2-3 and 4-8 controls. Bars represent mean and SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 by Mann-Whitney U test.

See also Figure S7.