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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Mamm Genome. 2016 Sep 6;27(11-12):556–564. doi: 10.1007/s00335-016-9662-7

Figure 1.

Figure 1

Mammary stem cell (MaSC) transplantation to evaluate gene function in mammary gland development and tumorigenesis. MaSCs are enriched from mammary glands removed from donor mice, transduced with high titer lentiviral vectors in cell culture, and transplanted into mammary fat pads of host mice from which mammary gland epithelia have been removed (A). Examples of resulting growth of mammary glands in host mice are shown; green fluorescence labeling due to EGFP expression with the lentiviral vector shows growth of transduced MaSCs (B); and histology demonstrates the presence of mammary gland tissue (B, see also figure 2).