Table 1.
Sequencing Platform |
Total output (bases per run) |
Total reads (million per run) |
Read Length (bases) |
Run time (days) |
Purpose/Definition | |
---|---|---|---|---|---|---|
Illumina | HiSeq X | 1.6 – 1.8 Tb | 6000 | 2×150 | < 3 | Allows sequencing of larger genomes (e.g. mammalian genomes) at population level |
MiSeq | 300 Mb – 15 Gb |
50 | 2×300 | 0.2 – 2.7 | Designed for particularly small genomes (e.g. bacterial genomes) and amplicon sequencing |
|
Life Technologies | Solid 5500 Systems |
80 Gb – 320 Gb |
1200 M – 2400 M |
50 – 2×50 bp |
7 | Offers application-per- lane sequencing that allows transcriptome, exome and genome sequencing concurrently in a single run. Additionally, pay- per-lane sequencing feature makes Solid 5500 Systems cost- effective because reagent usage does not require for unused lanes. |
Ion Torrent 520 Chip |
600 Mb – 2 Gb | 3 | 200 – 400 bp |
0.1 | Ion S5 System allows generation of diverse sequencing data ranging from targeted re-sequencing to genome sequencing with as little as 10 ng sample. |
|
Ion Torrent 540 Chip |
10 – 15 Gb | 60 | 200 – 400 bp |
0.1 | ||
PacBio | Sequel System |
500 Mb – 16 Gb |
55 – 880 M | up to 60 kb | < 0.1 – 0.3 | Useful in the studies of de novo assembly of large genomes. Sequel System can be utilized for generating variation, expression and/or regulation related sequencing data. |
PacBio RS II | 500 Mb – 16 Gb |
55 – 880 M | up to 60 kb | < 0.1 – 0.3 | Much more suitable for sequencing small genomes although animal and plant genomic studies is also possible. |
|
Nanopore | PromethION | up to 12 Tb1 | 1250 M1 | 230 – 300 kb1 |
2 | Ideal for large sample numbers. PromethION can sequence up to 48 samples in a single run |
MinION | up to 42 Gb1 | up to 4.4 M1 | 230 – 300 kb1 |
2 | Portable sequencing instrument. MinION can be run with a desktop or laptop computer and data can be performed in real time. |
Measured at “fast” mode in which 500 bases pass through the pore per second.