Table 2.

Capture technologies for WES

Platform	Target Capture Region Length	Bait Length	Bait Density	Notes
NimbleGen	64.1 Mb	Not Available	2.8 Mb	Requires 1–2 µg of DNA, utilizes overlapping biotinylated DNA probe design. Adapter addition is through ligation.
Agilent	51.1 Mb	66.48 Mb	1.63 – 3.5 Mb	Requires 2–3 µg of DNA, utilizes biotinylated cRNA as bait from non- overlapping probes that are directly adjacent to each other. Adapter addition is through ligation.
Nextera	62.08 Mb	33.01 Mb	1.44 Mb	Requires 50 ng of DNA. The gapped capture probes rely on paired-end reads to extend outside the bait sequences and to fill the gaps. Utilizes transposons for fragmentation and adapter ligation without the need of mechanical shearing.
TrueSeq	62.08 Mb	33.01 Mb	-	This capture platform from Illumina is similar to Nextera except that the input requirement is higher (1 µg) and ultrasonication is used for DNA fragmentation.