Skip to main content
. Author manuscript; available in PMC: 2017 Jul 5.
Published in final edited form as: Oncogene. 2016 May 16;36(1):60–70. doi: 10.1038/onc.2016.175

Figure 5. A specific inhibitor of SCFSkp2/Cks1 selectively inhibited DKO prostate tumor cells and DU145 cells in monolayer cultures.

Figure 5

(A) The designed inhibition mechanism for SCFSkp2/Cks1 inhibitor Compound 1 (C1) compared to p27T187A KI (p57 is a predicted substrate). (B) Proliferation chart showing cell numbers relative to the vehicle (DMSO) following treatment with Skp2/Cks1 pocket inhibitor Compound 1 (C1) at three concentrations after 2 days in monolayer cultures of the indicated cells. (C) p27T187A KI inhibited proliferation of DKO prostate tumor cells in monolayer culture (representative of three independent experiments). Error bars are s.e.m. of the means of triplet plates. p value is by two-sided t test. Cell numbers were counted in triplicate plates every two days for six days. (D) Western blot following treatment with C1 (5 μM) for 2 days.