Fig. 5.
FLLL32 inhibition of pSTAT3 (Y705) results in reduced cellular proliferation and increased apoptosis in UM-CHOR-1 cells. (A) Immunofluorescence staining was performed on UM-CHOR-1 cells treated with FLLL32 or with equal volume 0.1% DMSO vehicle as a control. Of cells treated with FLLL32, 16.1% exhibited positive staining compared with 62.7% in control-treated cells, showing a significant reduction in pSTAT3(Y) expression in the FLLL32-treated cells (p < 0.05). (*Statistically significant.) (B) Ki-67 staining was significantly higher in control-treated UM-CHOR-1 cells (57.1%) relative to FLLL32-treated cells (13.3%), indicating decreased proliferative activity with FLLL32 treatment (p < 0.05). (C) CC3 was significantly higher in FLLL32-treated UM-CHOR-1 cells (87.9%) relative to control-treated cells (22.2%), indicating increased apoptosis induced by FLLL32 treatment (p < 0.05). CC3, cleaved caspase 3; DMSO, dimethyl sulfoxide; pSTAT, phosphorylated signal transducer and activator of transcription.