FIG. 7.

ChABC digested extracellular BX-GAGs in glycocalyces. A BX-GAGs visualized in different cells and regions of the crista ampullaris before chABC digestion. B Remaining BX-GAGs following chABC. C Magnified region of tissue demonstrating final signal of BX-GAGs by subtracting fluorescence postdigestion (2), from predigestion (1). D Qualitative fluorescence levels of BX-GAGs in hair cells of the utricle were not effected by either chABC (i) or hepI, hepII, hepIII (ii). E, Capillaries supplying the saccular macula developed BX-GAG fluorescence in the glycocalyx and was eliminated following 18-h digestion with chABC. HepI, hepII, hepIII partially reduced BX-GAG signal in the capillary glycocalyx