Fig. 8.
A Six-degree-of-freedom pharmacokinetic model including (C 1) xyloside (BX) delivery into the extracellular space, (C 2) reversible BX entry into the cytoplasm, (C 3) reversible BX entry into the Golgi, (C 4) Michaelis-Menten kinetics to produce BX-GAG in the Golgi with BX core, (C 5) BX-GAGs exit the Golgi, (C 6) BX-GAG exits the cell and localizes in the extracellular glycocalyx, and (C 6) BX and BX-GAGs is cleared from the extracellular space through metabolism. B Simulated concentrations of either BX or BX-GAGs as functions of time. In silico, BX was rapidly taken up by the cytoplasm (C 2 + C 3) and Golgi (C 4), ultimately priming BX-GAG (C 5). BX-GAGs migrated to extracellular glycocalyces (C 6), reaching a plateau 5–7 h after the initial bolus injection