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. 2016 Nov 17;7:1864. doi: 10.3389/fmicb.2016.01864

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Copyright © 2016 Qin, Sang, Ren, Zhang, Li, Cui and Yao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of acetylation of K36 in vivo. (A) The mRNA levels of Lrp-regulated genes in chromosome lrp mutants. Bacteria were grown to log phase, and harvested to isolate total RNA. The transcriptional levels of target genes were determined by qPCR with the methods of 2^-ΔΔCt. The expression of the tested gene was normalized to that of 16S rRNA and compared to eWT. ^∗P < 0.05, ^∗∗P < 0.01, Student’s t-test. (B) Mannose-sensitive yeast agglutination assay. 5 μl the chromosome lrp mutants culture were mixed with equal volume of bakers’ yeast suspension (10 mg/ml) on slide. Mannose inhibition of agglutination was confirmed using 3% α-D-mannose in the yeast suspension.